Nitrocefin is a chromogenic cephalosporin substrate, developed by Glaxo Research Limited coded 87/312; 3-(2,4 dinitrostyrl) – 6R, 7R) -7-(2 thienylacetamido)-ceph-3-em-4 carboxylic acid, E-isomer. This compound exhibits a rapid distinctive colour change from yellow to red as the amide bond in the beta- lactam ring is hydrolysed by a beta-lactamase (E.C.18.104.22.168.).It is often used as as an indentification stick. It is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria. It is used to detect the presence of beta-lactamase enzymes produced by various microbes.
In Microbiology Nitrocefin is used as a disk for rapid detection of beta-lactamase production in Haemophilus spp., N. gonorrhoeae, M. catarrhalis, staphylococci, enterococci, and anaerobes.
Antibiotics & Antibacterials
|Appearance||yellow to orange solid|
|MSDS Data Sheet||Click to download|
|MDL Number||Request if not shown|
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