MES [2-(N-Morpholino)-Ethanesulfonic Acid]
A zwitterionic buffer effective in the pH range of 6-8, used in various protein characterization assays.MES is a zwitterionic N-substituted aminosulfonic acid known as a Good’s buffer, active in the pH range 6-8 with a pKa of 6.10 at 25°C. With a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. MES is used as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide.
Product Details
| Category |
Biological Buffers Microbiology Products |
|---|---|
| Price | POA |
| CAS Number | 4432-31-9 |
| Formula | C6H13NO4S |
| Molecular Weight | 195.24 |
| Purity | 99.5% |
| Appearance | crystalline powder |
| Availability | In Stock |
| Storage Conditions | Room temperature |
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