Bicine Buffer (N,N-BIS(2-Hydroxyethyl)Glycine)
Bicine is widely utilized as a buffering agent for low temperature work. It is used in kinetic study of a quaternary transition-state analogue complex of creatine kinase reaction. It is employed in the thin layer ion exchange chromatography method for protein resolution.
It also used Western blotting, also called protein blotting or immunoblotting. It uses antibodies to identify specific protein targets bound to a membrane; the specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture, such as cell or tissue lysate.
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