ADA Buffer (N-(2-Acetamido)iminoacetic acid) is a zwitterionic organic chemical buffering agent; one of Good’s buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/ml.
ADA has been used in a study to describe the application of scanning capacitively coupled contactless conductivity detection (SC(4)D) for the determination of pH dependant behaviour of two aminopolycarboxylates immobilised onto the surface of a monolithic capillary column.
It is frequently used in the field of hematology in the field of lysing reagents and blood counter formulations. used in the production of an isotonic multipurpose blood diluent,often used in automatic particle counting systems.
An example would be as a component in an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.
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|Storage Conditions||Room temperature|
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