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5-Bromo-6-Chloro-3-Indolylphosphate-P-Toluidine Salt (Magenta Phosphate P-Toluidine Salt)

5-Bromo-6-chloro-3-indoxyl phosphate p-toluidine salt, also known as Magenta-phos p-toluidine salt, is a histochemical substance used for visualizing areas of alkaline phosphatase activity. Hydrolysis of the phosphate group on 5-Bromo-6-chloro-3-indoxyl phosphate by alkaline phosphatase generates an insoluble indigo compound. The indoxyl species resulting from phosphate hydrolysis of related compounds can be reacted with tetrazolium salts, reducing the tetrazolium to a colorful insoluble formazan species. A solubilization technique involving reduction of the insoluble hydrolytic product of the closely related 5-Bromo-4-chloro-3-indoxyl phosphate permitted the use of this substrate in quantitative ELISA experiments. 5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt is a substrate of TNAP.

Product Details

Category Biochemicals & Diagnostics
Enzyme Substrates
Microbiology Products
Price POA
CAS Number 6769-80-8
Formula C8H6BrClNO4P · C7H9N
Molecular Weight 433.62
Purity 97.0%
Appearance white to off-white powder
Availability In Stock
Storage Conditions -18° C

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