5-Bromo-6-Chloro-3-Indolylphosphate-P-Toluidine Salt (Magenta Phosphate P-Toluidine Salt)
5-Bromo-6-chloro-3-indoxyl phosphate p-toluidine salt, also known as Magenta-phos p-toluidine salt, is a histochemical substance used for visualizing areas of alkaline phosphatase activity. Hydrolysis of the phosphate group on 5-Bromo-6-chloro-3-indoxyl phosphate by alkaline phosphatase generates an insoluble indigo compound. The indoxyl species resulting from phosphate hydrolysis of related compounds can be reacted with tetrazolium salts, reducing the tetrazolium to a colorful insoluble formazan species. A solubilization technique involving reduction of the insoluble hydrolytic product of the closely related 5-Bromo-4-chloro-3-indoxyl phosphate permitted the use of this substrate in quantitative ELISA experiments. 5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt is a substrate of TNAP.
Product Details
| Category |
Biochemicals & Diagnostics Enzyme Substrates Microbiology Products |
|---|---|
| Price | POA |
| CAS Number | 6769-80-8 |
| Formula | C8H6BrClNO4P · C7H9N |
| Molecular Weight | 433.62 |
| Purity | 97.0% |
| Appearance | white to off-white powder |
| Availability | In Stock |
| Storage Conditions | -18° C |
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