3,3′-Diaminobenzidine tetrahydrochloride hydrate is a water-soluble preparation of 3,3′-Diaminobenzidine, a tetraamine biphenyl compound used as a histological stain for peroxidase activity. Reaction between 3,3′-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
Biochemicals & Diagnostics
|Formula||C12H14N4 · 4HCl · xH2O|
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