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MES [2-(N-Morpholino)-Ethanesulfonic Acid]

A zwitterionic buffer effective in the pH range of 6-8, used in various protein characterization assays.MES is a zwitterionic N-substituted aminosulfonic acid known as a Good’s buffer, active in the pH range 6-8 with a pKa of 6.10 at 25°C. With a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. MES is used as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide.

Product Details

Category Biological Buffers
Microbiology Products
Price POA
CAS Number 4432-31-9
Formula C6H13NO4S
Molecular Weight 195.24
Appearance crystalline powder
Purity 99.5%
Storage Conditions Room temperature

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