Coomassie® Brilliant Blue R-250 (C.I. 42660)
Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. Coomassie Brilliant Blue G-250 differs from Coomassie Brilliant Blue R-250 by the addition of two methyl groups. Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. Coomassie Blue R-250 is a triphenylmethane dye used extensively in SDS-PAGE for the analysis of proteins.
Dyes, Stains & Indicators
|Storage Conditions||Room temperature|
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